FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3570962
Sequences flagged as poor quality0
Sequence length20-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95990.2688071169617599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG12550.030.289371
TACACTG19950.029.397935
GTGTAGG27100.026.4509051
TATACTG20050.025.459475
TACACCG27500.024.8813385
TAGGACC3702.0536645E-623.4830324
TAGACTG13000.023.3927125
CCTACAC24300.023.2414383
GTCTAAG12000.022.626881
CTACACT23550.022.5980554
GTATATA16700.022.1120151
GTGTATA12400.021.896981
CTATACT16500.021.7218044
GTATAAG17800.021.3557051
GGCTCTT30856.37192E-821.338732245
ACACTGT16300.021.3220166
TATAGGG10400.020.8863492
GTATATG13650.020.6874311
TCTACAC31100.019.9058343
GCTACAC26900.019.78383
Produced by FastQC (version 0.11.5)