FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3570962
Sequences flagged as poor quality0
Sequence length20-251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACG20252.515906E-620.687185245
GGAGTGT13803.8465987E-720.237463245
GTCCTAG7400.019.7475281
CCTATAC10900.019.439543
TATACTG17100.019.2278585
TAGACTG11050.018.5144255
CCCCTTT45351.75546E-518.474773245
GTGTAGC17500.018.3708461
GTGTAGA21150.017.2732521
GTATATG15700.017.2193411
GTCTAGG10400.016.8613511
AGACTGT16950.016.8116136
TAGACCG12250.016.7007685
GTGTAGG17500.016.7007681
CTATACG7451.8189894E-1216.6727474
GTACAGG21650.016.5368461
ACCTATG17050.003054147116.379883245
TACACTG13400.016.3580275
GTGTATG14750.016.3469371
GGCCCGG86451.1775101E-616.152514245
Produced by FastQC (version 0.11.5)