FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872507
Sequences flagged as poor quality0
Sequence length20-251
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79530.27686616603545267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCTT26800.050.630447245
TGTTAGG9155.6500215E-429.65893245
GTCCCTT24957.2759576E-1229.005123245
CTTAGGT5100.028.5493323
TATACAG10450.027.8663335
TACACCG27150.024.1328625
TATACTG8300.023.807495
ACACTGT7900.023.6964636
TACACTG7500.023.5735915
GCTTAGG7808.723493E-623.194803245
GTATAGG11700.023.1113661
TATACCG14000.023.0288245
CTACACC29800.022.6848134
CTCTATA7450.022.3358152
CTATACT5605.0931703E-1022.285964
TAGATAG7500.022.186915
GTGTAGA13600.022.1767141
TATAGTG4704.354115E-822.1279035
CCCTATA9300.021.2475452
TAGGACA7850.021.1976854
Produced by FastQC (version 0.11.5)