FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872507
Sequences flagged as poor quality0
Sequence length20-251
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG51250.029.538116245
GGGGGGG252250.029.460936245
TTTGGGG20753.5011838E-426.529303245
TTAAGGG10555.2502856E-426.089245245
CCGGGGG129500.021.254171245
GGGCGGG167050.020.595745245
CGGGGGG140850.020.51854245
GCGGGGG151100.020.037437245
TACACCG19750.020.0308865
GGGGTGG63000.019.660109245
CCCCGGG123050.019.013027245
GGTTGGG32202.1232033E-417.095747245
GGCCCTT41202.9831426E-1016.70155245
AAAGGGG33003.0603784E-416.681303245
CCGTGTG16600.001722796216.580814245
AGAACCG25201.6110134E-616.383425245
GTTCTAA8858.859599E-515.550369245
GGGCCCG115450.015.4964905245
GGAGGGG73509.7817465E-514.979131245
ACCTTAA9250.001400592714.877919245
Produced by FastQC (version 0.11.5)