FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651497
Sequences flagged as poor quality0
Sequence length20-251
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56280.3407817271239367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAA6707.9460925E-443.885056245
CTAGGAC1357.223234E-540.5213743
CTTACAC3052.5102054E-1035.871383
GTCTAGG1652.3520825E-433.153851
TACAGGG5251.8885694E-628.002844245
TCTACAC11150.027.4745793
GTGTAGA11000.026.8546181
GTGTAGG9000.026.7441041
TACACCG22300.026.4933435
GTGTAAG3001.0173098E-525.5284611
CTAGGGT2200.001266002524.8653874
TAGACCG7600.024.4727755
CTACACA3651.7109032E-623.9797694
CCCTATG6151.525984E-423.904865245
GTGTATA3251.8912957E-523.5647371
GCTAAGA2801.8682133E-423.4445082
GTATAAG3302.1283655E-523.2076951
CCTTACA3352.3907569E-522.8613112
TTACACC6406.002665E-1122.2234384
GTACTGT3002.9802322E-421.8815386
Produced by FastQC (version 0.11.5)