FastQCFastQC Report
Mon 27 May 2019
final_pure_reads_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefinal_pure_reads_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651497
Sequences flagged as poor quality0
Sequence length20-251
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTAT6950.006070546354.29175245
TGGGGGG35255.456968E-1239.249214245
CCTTTAG3750.001475909133.540237245
GTGTGGG16951.4454614E-429.68163245
CAGGGGG24159.6028694E-426.04056245
GGGGGGG165300.024.34863245
ATACTGG5400.0018531823.291832245
CCCAAAA11350.002850322522.163153245
TTAAAAA30750.003913567420.451365245
CCCAAGG18750.002103312920.124144245
TACACCG13150.019.0040235
TGTGTAC4554.156391E-918.8309883
GGGGGAG42250.001158354717.861666245
TTAGGGC2851.8363644E-417.5370463
GCGGGGG101052.1100277E-1017.425655245
CGGGGGG82950.016.679142245
TGTACAG6004.1836756E-1016.6601945
GTTATGC3700.001333858116.64824185-189
ACACCGT14800.015.9204566
GCGGTGG55653.9781278E-415.820868245
Produced by FastQC (version 0.11.5)