FastQCFastQC Report
Tue 25 Oct 2016
1_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16510085
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT7900.022.42762113
CGAACTA29400.020.8664384
TAAGCAG94950.020.2386519
AACCGGT97200.019.32152438
ACCGGTC95700.019.26206439
CTGAGTT98750.018.87708318
CTGACTT95400.018.86863158
CGTAAGC103350.018.5927547
TCTGACT98350.018.47647557
GTTCTGA103200.018.23028415
AGTTCTG105150.017.89182714
TCTGAGT107650.017.74609817
CCGGTCC105750.017.43307140
GTAAGCA114200.017.3335158
CTAGGTT10400.017.14547114
CCCCGAT53650.016.834081
GAGTTGG113950.016.81693820
AAACCGG114650.016.63327837
GAACTAC38400.015.9761575
CCGTAAG115050.015.9471656
Produced by FastQC (version 0.11.5)