FastQCFastQC Report
Tue 25 Oct 2016
1_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16510085
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT527750.053.1608771
ACAGTTT668750.042.2220882
TTGATAC241950.041.3351633
TGATACA251450.039.727424
ATTGATA264150.037.9258422
GATACAG263350.037.8461385
ATACAGT268550.037.1976556
CAGTTTC832000.033.931593
TATTGAT353950.030.0206971
AGTTTCT1034950.027.3168964
GTTTCTG1425700.025.5353571
TTCTGTA1467600.024.6065853
TCTGTAC1479400.024.3437734
TGTACTA1546600.023.2859636
CTGTACT1569000.022.958395
TACTATA1569900.022.9244848
TTTCTGT1596800.022.8325252
GTACTAT1589150.022.6566937
ACTATAT1601200.022.4850929
CTATATT1654200.021.85717210
Produced by FastQC (version 0.11.5)