FastQCFastQC Report
Tue 25 Oct 2016
1_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37850
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC152.821596E-4117.4819231
CGGGTAG206.1543815E-6116.856215
ATCGGTA152.913057E-4116.545812
AGCGTCG550.0105.9507410
TAGGGTA358.1527105E-9100.429919
TCGGGTA358.305506E-9100.16244514
GATGTGC251.8897756E-593.236666
GCGTCGT501.0913936E-1193.2366611
GATCGTA251.9023008E-593.1131
GATCGGT251.9023008E-593.1131
TCTCGGT454.2564352E-1091.6201436
TCAATCT208.9522783E-487.8761718
GTCTAGG208.9999515E-487.75916
TCTTGTA209.0478145E-487.6421514
AGCATCG209.1441104E-487.4093610
GATCGGA2000.087.2934341
TGTGTAG552.3646862E-1184.9863215
TCGGAAG1100.084.760593
TAGGGAA900.084.621519
TCCTGTA359.794003E-783.46870414
Produced by FastQC (version 0.11.5)