FastQCFastQC Report
Tue 25 Oct 2016
2_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16877079
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC100200.022.65485639
TAAGCAG106100.022.2868829
AACCGGT102300.022.13215438
CGTAAGC107300.021.6602277
CTGACTT102450.021.54581658
CTGAGTT107500.021.35479218
TCTGACT104050.020.9835557
AGTTCTG110950.020.84517914
GTTCTGA112400.020.42263415
GTAAGCA120450.020.0620448
GCTAGGT9150.019.653946113
TCTGAGT116950.019.62837217
CCGGTCC114950.019.54860540
GAGTTGG122150.018.98356620
AAACCGG119950.018.92289437
CCGTAAG120700.018.3475366
CGAACTA37450.018.0167944
CTAGGTT10550.017.818678114
AGTTGGC130950.017.71073321
GGTCCCT131050.017.2838442
Produced by FastQC (version 0.11.5)