FastQCFastQC Report
Tue 25 Oct 2016
2_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8048
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT160.19880715705765406No Hit
GCTCTTCCGATC130.16153081510934394Illumina Single End Adapter 1 (100% over 12bp)
GCGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGTCTGATGGAACGACGA90.11182902584493042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGA100.009417717130.8872286
AAAGGTT100.00963256129.9104583
Produced by FastQC (version 0.11.5)