FastQCFastQC Report
Tue 25 Oct 2016
2_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16877079
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT574650.050.5033071
TTGATAC278100.043.708373
TGATACA282050.042.993734
ATTGATA299150.040.6700332
ACAGTTT719800.040.6253742
GATACAG301150.040.250385
ATACAGT304500.039.8062636
TATTGAT379150.033.45671
CAGTTTC880650.033.147313
AGTTTCT1072450.027.3642274
GTTTCTG1459500.025.0070611
TTCTGTA1512800.023.9523643
TCTGTAC1519700.023.7827324
TGTACTA1576500.022.9302656
TACTATA1597250.022.6780728
CTGTACT1595650.022.6485795
TTTCTGT1633000.022.372282
GTACTAT1616050.022.3701487
ACTATAT1631350.022.1946099
ACATAAG52750.021.8735966
Produced by FastQC (version 0.11.5)