FastQCFastQC Report
Tue 25 Oct 2016
2_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12342
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG300.24307243558580457No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA250.20256036298817048No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT240.19445794846864364No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG180.14584346135148274No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC160.12963863231242911No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA150.12153621779290229No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA150.12153621779290229No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA150.12153621779290229No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA140.11343380327337546No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG140.11343380327337546No Hit
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA130.10533138875384865No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT130.10533138875384865No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA130.10533138875384865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG100.0087523134.475107
GCGCGGG100.0087523134.475109
CGCGCGG100.0087523134.475108
TTTCGCG100.008884416133.80597105
CATGCCA100.008884416133.80597104
GGTTTCG100.009017848133.14357103
GACCGAG100.009017848133.14357103
GGACCGA100.009152606132.48769102
CGGTTTC100.009152606132.48769102
CCGGTTT100.009152606132.48769101
GCCGGTT100.009288695131.83824100
AACAGTT100.009288695131.83824100
CACGCCG100.009426121131.1951397
TTTCACC100.009705009129.9275491
CTACGAA100.009705009129.9275494
TCACCAC100.009705009129.9275493
ACTCTTT100.009989323128.684289
AACTGGC300.005602330455.1127055
TTTATGG350.00981084247.82750731
Produced by FastQC (version 0.11.5)