FastQCFastQC Report
Tue 25 Oct 2016
3_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15370170
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT9450.030.398493113
CTAGGTT10450.028.314377114
CCCCGAT45900.019.6588061
CCTATGG36200.018.3921667
TAAGCAG81250.018.0796139
TATCCTA37100.017.90961864
CGAACTA36950.017.3724274
TGGACTA27750.017.293066118
TCCTATG39500.017.28678566
TAGGTTA16900.017.210184115
ACCGGTC79550.017.10626239
GGACTAC27550.017.020782119
CTGACTT81900.016.36239858
CTAAGTA19250.016.22556315
AGCTAAG18750.016.04074913
CTGAGTT87200.015.85453418
CTATGGG43900.015.84356968
AACCGGT85600.015.82793638
CGTAAGC90300.015.6905727
AGGTTAG18300.015.613027116
Produced by FastQC (version 0.11.5)