FastQCFastQC Report
Tue 25 Oct 2016
3_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15370170
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT497650.049.8218731
TTGATAC241500.042.9504473
TGATACA250850.040.9810944
ATTGATA257750.040.3989332
ACAGTTT625600.039.8451842
GATACAG268050.038.5464485
ATACAGT267450.038.4183586
CAGTTTC777200.032.0140953
TATTGAT340050.031.8800241
AGTTTCT956200.026.0577164
GTTTCTG1291150.025.0274221
TTCTGTA1354150.023.7014143
TCTGTAC1360000.023.565744
TGTACTA1415450.022.6685546
TACTATA1433600.022.3836738
CTGTACT1435000.022.3345175
GTACTAT1452550.022.0823947
TTTCTGT1466650.022.0604532
ACTATAT1462250.021.9660369
CTATATT1505100.021.38330310
Produced by FastQC (version 0.11.5)