FastQCFastQC Report
Tue 25 Oct 2016
3_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14257
Sequences flagged as poor quality0
Sequence length2-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT250.17535245844146735No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG210.14729606509083257No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA180.1262537700778565No Hit
GTCACACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCG170.1192396717401978No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA170.1192396717401978No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG150.1052114750648804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT100.00874583134.60852108
ACAAACC100.008858017134.03815116
TTCGCCA100.008858017134.03815113
GCTTCGC100.008858017134.03815111
CTCGACC100.009551266130.7148797
GTGGGTG153.3000615E-4112.5729526
CTGTGTA250.002546761467.3042510
TCGGAAG250.002656469666.595793
TACAGTT300.005469505755.496491
TCTTTAC300.005469505755.496494
Produced by FastQC (version 0.11.5)