FastQCFastQC Report
Tue 25 Oct 2016
4_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14798981
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT11700.040.980698113
CTAGGTT12900.037.94842114
TAGGTTA16750.028.177315115
AGGTTAG19700.024.469347116
GCGCTAG19000.024.203362111
CGCTAGG20600.022.791624112
CTAAGTA20500.022.29568115
GACTAGG15350.022.185438109
TAAGGGG41800.021.29489971
AGCTAAG21250.020.96361213
CTAGGCT15650.020.741838111
TGGACTA30650.019.86412118
GGACTAC31350.018.875767119
CCCTTAG27800.018.16033293
GGTTAGG27700.018.024515117
CCCCGAT43900.017.7915521
ACGCCTA32650.017.35887768
TAGGGTA25900.016.9804620
TGACTAG20100.016.843258108
TAAGCAG71850.016.5859189
Produced by FastQC (version 0.11.5)