FastQCFastQC Report
Tue 25 Oct 2016
4_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111
Sequences flagged as poor quality0
Sequence length2-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAACA100.008550939135.29999119
CAATACG100.008723625134.40398118
GACCATT100.009255545131.78572113
GCAACGG100.009437524130.93549109
CCGGTGG153.3134068E-4112.127072
TGCTGCA300.003416528562.25459779
Produced by FastQC (version 0.11.5)