FastQCFastQC Report
Tue 25 Oct 2016
4_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14798981
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT334750.058.6679571
ACAGTTT458850.043.078712
CAGTTTC551450.035.834763
TGATACA138800.032.6438264
TTGATAC142050.031.814843
GTTTCTG1049900.031.6740021
GATACAG143900.030.9641175
ATACAGT145050.030.6004896
TCTGTAC1117400.029.4097924
TTCTGTA1120000.029.4080923
TGTACTA1162850.028.2567656
AGTTTCT706550.028.1327764
CTGTACT1179350.027.8354855
TACTATA1179600.027.833548
ATTGATA162700.027.7410322
GTACTAT1194050.027.5243937
ACTATAT1212000.027.1338259
TTTCTGT1228600.027.0249922
CTATATT1250500.026.35460310
ACATAAG58400.025.2684216
Produced by FastQC (version 0.11.5)