FastQCFastQC Report
Tue 25 Oct 2016
4_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG160.16061031921300944No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG150.15057217426219635No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG130.13049588436057016No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA120.12045773940975708No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG120.12045773940975708No Hit
CTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAA110.11041959445894399No Hit
GTGCTCTTCCGATC110.11041959445894399Illumina Multiplexing Adapter 1 (100% over 14bp)
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA100.10038144950813091No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA100.10038144950813091No Hit
ATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGT100.10038144950813091No Hit
GTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG100.10038144950813091No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT100.10038144950813091No Hit
CTCTACTTGTCTAAGGGGCAGCAGGACATACCAGTGGTGTGTGGATCAGA100.10038144950813091No Hit
ATCGAACTGGCAGAATCTGCCCTTGCTCCATTTATGGCCTTTGACGGTGG100.10038144950813091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTT100.008201889137.25157115
ACCTTGT100.008201889137.25157118
CCATACC100.008201889137.25157117
CTCGTGG100.008201889137.25157117
GTTCTGC100.0085161505135.54659110
AGCGCAA100.008838309133.88344107
GGAATCA100.00900238133.06708106
TAAGAGA100.009168461132.2606104
GTCTCAA100.009336564131.46385100
CGAGCTA100.009336564131.46385101
TCTCAAG100.009336564131.46385101
ATGACTT100.009678886129.8988293
GATGACT100.009678886129.8988292
AGTTTCT250.002761588265.797992
GTTTCTG507.5997016E-443.865333
TTTCTGT801.8020766E-434.2697874
Produced by FastQC (version 0.11.5)