FastQCFastQC Report
Tue 25 Oct 2016
5_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14260556
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT12400.028.497887113
CTAGGTT12500.027.907465114
TAAGGGG46900.023.11538571
TGGACTA30350.020.595594118
TAGGTTA17000.020.251453115
GGACTAC31150.019.976519119
GCGCTAG17700.019.723953111
CGAACTA45250.019.351924
AGTAAGG58700.019.04930369
AGCTAAG19850.018.99231513
CCTATGG43450.018.84174367
CCCCGAT45400.018.782311
TAAGCAG76300.018.698899
GACTAGG14950.018.198257109
GTAAGGG60500.018.00687270
ACCGGTC74150.017.93833239
ACGCCTA33100.017.85300368
CTGAGTT75300.017.71754818
CTAGGCT14950.017.51408111
AGGTTAG20600.017.493258116
Produced by FastQC (version 0.11.5)