FastQCFastQC Report
Tue 25 Oct 2016
5_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14260556
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTAATATAAAAGGCTTTGGAAGGGGATTTTGGGGGACACCCGCCAGAT150370.10544469654619358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT330800.055.254511
ACAGTTT446650.041.2867552
TTGATAC152800.034.824053
TGATACA153600.034.6804544
GATACAG158550.033.6724475
ATACAGT155900.033.6479576
CAGTTTC554050.033.3051573
ATTGATA174750.030.3496172
GTTTCTG1021700.029.8563861
ACATAAG53300.028.5246
TTCTGTA1070850.028.232053
TCTGTAC1076450.028.0043184
CATAAGG54050.027.1635867
AGTTTCT695400.026.6105064
TGTACTA1133350.026.5883036
CTGTACT1148900.026.219355
TTTCTGT1164550.026.134342
TACTATA1155550.026.03978
GTACTAT1169600.025.7608137
ACTATAT1187300.025.3596299
Produced by FastQC (version 0.11.5)