FastQCFastQC Report
Tue 25 Oct 2016
5_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA140.16470588235294117No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG130.15294117647058825No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG130.15294117647058825No Hit
TACAGTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACT130.15294117647058825No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC130.15294117647058825No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA120.1411764705882353No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG120.1411764705882353No Hit
CAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATC110.12941176470588237No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG110.12941176470588237No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA110.12941176470588237No Hit
GTTTGAATCAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAA110.12941176470588237No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT100.1176470588235294No Hit
AGTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGA90.10588235294117646No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG90.10588235294117646No Hit
CCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGAC90.10588235294117646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG100.008341922136.35252113
GTCGTGT153.3823427E-4111.48823513
CGTCGTG153.3823427E-4111.48823512
AGGTTGA207.520811E-491.120275
CAGCAAT250.00217500669.7656556
AGCAATG300.00437113258.49691457
AACTTTG300.005299303755.74411815
GTTTCTG551.7697766E-550.676471
TTTCTGT701.2711789E-647.7806742
TTCTGTA701.2711789E-647.7806743
TCTGTAC602.9609619E-546.453434
TGTACTA654.7510413E-542.880096
CTGTACT654.7510413E-542.880095
GTACTAT707.356659E-539.8172267
TACTATA707.356659E-539.8172268
ACTATAT707.356659E-539.8172269
CTATATT751.1046992E-437.16274610
TATATTG751.1046992E-437.16274611
Produced by FastQC (version 0.11.5)