FastQCFastQC Report
Tue 25 Oct 2016
6_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488033
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC220650.14246483075029606No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT199860.1290415638964612No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG190440.12295944875633982No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA166030.10719889349409316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT21200.044.52856113
CTAGGTT22350.042.48225114
TAGGTTA27000.035.377228115
GCGCTAG29550.032.47709111
CGCTAGG29500.031.80293112
ACGCCTA58400.031.21665268
AGGTTAG31350.030.881742116
CTACGAC63150.029.40112772
AGCCCGA62350.029.2552559
AGCTAAG32050.029.19077113
GGTTAGG34350.029.163958117
TGTTACG49600.028.5842253
TAAGGGG93500.027.94351671
TAGGGTA34650.027.67587520
CTAAGTA34100.027.6075615
CCCGACC68000.026.97969261
TACGACC70250.026.0357573
GGACTAC71200.025.940386119
CCAGGTT101750.025.67330232
CCCAGGT103900.025.47670231
Produced by FastQC (version 0.11.5)