FastQCFastQC Report
Tue 25 Oct 2016
6_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488033
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT358960.23176603510594276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT308400.056.116211
TTACCAG112800.041.076563
ACAGTTT426450.040.8691672
TACCAGA126000.036.773784
CCAGATC126100.036.330986
ACCAGAT130800.035.0707475
TCCGGAC138400.033.86571511
ATACAGT139400.033.8239256
CAGTTTC522150.033.5020833
GATACAG144850.033.1139645
TTGATAC145100.032.7340353
CAGATCC142300.032.4825867
TGATACA146050.032.362314
CTTTACC152700.031.6363121
GTTTCTG993200.031.0039561
GATCCGG151350.030.5425559
ATCCGGA158700.029.53304510
ACATAAG53250.028.6053716
ATTGATA166900.028.5969092
TTCTGTA1070750.028.5589713
Produced by FastQC (version 0.11.5)