FastQCFastQC Report
Tue 25 Oct 2016
6_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9341
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT310.3318702494379617No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCTGCGAAATCTCGTA150.16058237876030404No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG130.13917139492559683No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA110.11776041109088962No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC110.11776041109088962No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA100.10705491917353603No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA100.10705491917353603No Hit
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA100.10705491917353603No Hit
CCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGAC100.10705491917353603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTG100.009245177131.8461699
CTCCATC100.009245177131.84616102
AATTAAG100.009245177131.84616100
TCCATCA100.009245177131.84616103
GTACTCG100.0094245905131.0063697
CCGTACT100.0094245905131.0063695
CGTACTC100.0094245905131.0063696
TACTCGT100.0094245905131.0063698
TCTGTAC300.00537312255.589198
ACTATAT300.00537312255.5891913
CTGTACT350.00988465247.6478779
TACTATA350.00988465247.64787712
Produced by FastQC (version 0.11.5)