FastQCFastQC Report
Tue 25 Oct 2016
7_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15240137
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT451760.29642778145629534No Hit
CTGCCAGTAGTCATATGCTTGTTTCAAGGACTTAGCCATGCATGCCTCAG167620.10998588792213614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT306050.055.702051
TTACCAG124050.045.606443
ACAGTTT418350.041.000222
TACCAGA138300.040.9502334
CCAGATC142300.039.4747856
ACCAGAT145000.039.1388135
CTTTACC159100.036.7268141
TCCGGAC159750.035.17021611
CAGATCC158950.035.157777
ATACAGT138300.034.7781376
TTGATAC138950.034.403713
TGATACA140050.034.0927964
GATACAG141950.033.7597855
CAGTTTC510000.033.6779063
GATCCGG172450.032.510079
ATCCGGA176300.031.93308610
GTTTCTG953400.030.7173061
ATTGATA159700.030.3335822
AGATCCG185500.030.0962148
ACAGGGT188550.030.05286216
Produced by FastQC (version 0.11.5)