FastQCFastQC Report
Tue 25 Oct 2016
7_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8580
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA150.17482517482517482No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG130.15151515151515152No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG110.1282051282051282No Hit
ATCTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATG100.11655011655011654No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT100.11655011655011654No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG90.1048951048951049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG100.008301845136.56523119
ATAACAC100.008301845136.56523119
AGCTTCT100.008484299135.58275118
AGGGATG100.008484299135.58275116
CAGCTTC100.008484299135.58275117
AGCGGCT100.008669397134.61429107
CAAGAGG100.009240742131.790296
TGATTAC100.009635202129.9724191
TGGATTT100.009635202129.9724189
GATCGGA300.005547784355.1052631
Produced by FastQC (version 0.11.5)