FastQCFastQC Report
Tue 25 Oct 2016
8_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13737916
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT204420.1487998616384028No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG201590.1467398694241543No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC186190.13553001779891505No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG154190.11223681961659979No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA150980.10990022067393627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT14000.037.683464113
CTAGGTT15200.035.39982114
ACGCCTA52500.031.2853868
TGTTACG50000.030.4317843
TAGGTTA17700.029.81924115
CTACGAC58800.028.33948372
AGCCCGA58750.027.18696459
GTTACGA58100.026.7838654
TAAGGGG58800.026.12505371
CCCGACC62450.026.10091261
GCGCTAG20350.025.245697111
AGGTTAG21350.025.229374116
TACGACC68400.024.64840173
GGACTAC71550.024.47613119
TTACGAC63750.024.4102555
TGGACTA72350.024.13595118
CTTGTTA68100.023.1968921
AAGCCCG71500.022.89908258
CGCTAGG23300.022.789177112
GCGCCTA71750.022.76422742
Produced by FastQC (version 0.11.5)