FastQCFastQC Report
Tue 25 Oct 2016
8_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13737916
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT364040.2649892458215642No Hit
CTGCCAGTAGTCATATGCTTGTTTCAAGGACTTAGCCATGCATGCCTCAG139370.10144915720841502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT359250.048.755811
TTACCAG104800.043.3517533
TTGATAC178900.042.766363
TGATACA185950.041.2996674
ATTGATA191250.040.155012
ATACAGT192900.039.8131376
GATACAG198600.038.756145
TACCAGA118700.038.323474
ACAGTTT460100.038.2946472
CCAGATC125400.035.9553536
ACCAGAT125750.035.9456755
TCCGGAC131850.034.2480311
CTTTACC141350.032.549361
CAGATCC138300.032.5192877
CAGTTTC572500.030.9074423
TATTGAT267600.030.7233641
GATCCGG149350.030.4645069
ATCCGGA151950.029.90647910
AGATCCG151550.029.792228
AGTAGTC67900.028.9560266
Produced by FastQC (version 0.11.5)