FastQCFastQC Report
Tue 25 Oct 2016
8_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename8_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8815
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG150.17016449234259784No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC130.1474758933635848No Hit
CTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGA120.13613159387407828No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG120.13613159387407828No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCTGCGAAATCTCGTA110.12478729438457174No Hit
GTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG110.12478729438457174No Hit
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG110.12478729438457174No Hit
CTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGG110.12478729438457174No Hit
GTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG100.11344299489506524No Hit
GCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGA90.10209869540555871No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG90.10209869540555871No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA90.10209869540555871No Hit
GTGGAGGGCAACAAGTGCAACGGGTATCCGCAAATGACGGGGCGGCCAGA90.10209869540555871No Hit
CCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGAC90.10209869540555871No Hit
GTTTCTGTACTATATTGTATGACGCTGTCTTGGCGAGGAGCATGATGCGT90.10209869540555871No Hit
ATCGAACTGGCAGAATCTGCCCTTGCTCCATTTATGGCCTTTGACGGTGG90.10209869540555871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCCG100.008880335133.57535120
CCTGTGT100.0090646455132.66667108
GCAGTAC100.0090646455132.66667108
AGTTTCT252.3254514E-588.6454541
ATCGGAA305.5826145E-574.293332
TGTACTA305.5826145E-574.293337
GATCGGA305.7443773E-573.871211
GACAAGG250.002139998870.0670756
CTATATT351.1978988E-463.6811
GTACTAT351.1978988E-463.688
TTCTGTA351.1978988E-463.684
TCTGTAC351.1978988E-463.685
CTGTACT351.1978988E-463.686
TACTATA351.1978988E-463.689
ACTATAT351.1978988E-463.6810
TGGATGG300.003903711260.19135763
GTTTCTG402.3186166E-455.722
GGACAAG350.00811196650.04790555
TTTCTGT454.1480229E-449.528893
TATATTG506.9739507E-444.57599612
Produced by FastQC (version 0.11.5)