FastQCFastQC Report
Wed 5 Oct 2016
1_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6831
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCCGATC270.3952569169960474Illumina Single End Adapter 1 (100% over 12bp)
CCGAAATAAGCCATTTGTTTTCGGAACGATAAAACTCACGTCAGTTTGTG70.10247401551749377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACCC100.008671647134.37169118
TCCTAAC100.008905137133.193114
CCTAACA100.008905137133.193115
TTCCTAA100.008905137133.193113
CTAACAA100.008905137133.193116
TAACAAA100.008905137133.193117
TTTCCTA100.009142765132.03479112
ACTTTCC100.009142765132.03479110
Produced by FastQC (version 0.11.5)