FastQCFastQC Report
Wed 5 Oct 2016
1_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16409574
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT294850.064.537251
ACAGTTT428450.044.941192
CAGTTTC519550.037.0396353
GTTTCTG1084900.034.8766021
TTCTGTA1143550.032.9072233
TCTGTAC1149450.032.713434
TGTACTA1230300.030.6298986
CTGTACT1241300.030.2989395
TTTCTGT1250050.030.2835242
TACTATA1261000.029.8723288
GTACTAT1279850.029.4448537
ACTATAT1304500.028.8775229
AGTTTCT689150.027.9328274
CTATATT1357450.027.8121710
TATATTG1443850.026.24533811
ACATAAG72050.024.588376
CATAAGG74200.023.1743077
ATATTGG516650.022.94430412
GATAAAC76200.022.8677461
TGATACA109200.021.2597794
Produced by FastQC (version 0.11.5)