FastQCFastQC Report
Wed 5 Oct 2016
1_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename1_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12363
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTGTACTATATTGTTATAAGGTGCTCTCGGCTAAAATATACTTGAA190.15368438081371835No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA140.1132411227048451No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT140.1132411227048451No Hit
CGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCTCCA130.10515247108307045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTGA100.009965206128.8110799
CCATGGG300.002958337364.703705113
GTCTAAG402.1845875E-456.58299614
GTTTCTG550.001050748741.1512681
TTTCTGT600.00161181337.7222
TTCTGTA650.00238780534.8203053
TCTGTAC650.00238780534.8203054
GTACTAT750.004813792630.1775977
TGTACTA750.004813792630.1775976
CTGTACT750.004813792630.1775975
TACTATA800.006599426728.2914988
CTATATT850.0088721126.62729310
ACTATAT850.0088721126.6272939
Produced by FastQC (version 0.11.5)