FastQCFastQC Report
Wed 5 Oct 2016
2_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15614151
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC404850.2592840302364182No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG282190.1807270853215138No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA223540.1431650046166455No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG197180.12628288275167826No Hit
ACCGCACGCTCCACGTCTTGTGGTGCCATTCCGTCAATTTCTTTAAGTTT188080.12045483612909853No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT187140.11985281812632656No Hit
CTCTAATCATCTTCGATCCCCACACTTTGGTTCTTGATTGAGGAAGGTAT184380.11808519079903863No Hit
CCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTG180840.11581801661838674No Hit
GTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTC177400.11361488690611483No Hit
CTTCGCCAAATATGAGACCACACGAAGAAGAAGGAGCGCTTTGTCCCTCC175760.11256455762468288No Hit
ACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTCAAG172190.110278170103517No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC169520.10856818279777107No Hit
CGTAAATTTAATATATTCATTGGCGAGACGAGGAACGGGATGAAAAATAA163720.10485360363173125No Hit
CTCGCAATTTCCAGCTAAGTAGGGTAAAACCAACCTGTCTCACGACGGTC163450.10468068356710525No Hit
CTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCG160960.10308597630444333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT37800.057.182148114
GCTAGGT37950.056.41233113
TAGGTTA43350.049.85492115
GCGCTAG45800.046.1573111
CGCTAGG47450.045.540466112
GGTTAGG49450.044.571945117
AGGTTAG48800.044.442207116
AGCTAAG48850.043.09932713
CTAAGTA50300.042.2053915
TAGGGTA53800.039.0321920
CCCTTAG55900.038.42022393
GGCGCTA55800.038.01652110
TTAGGAA59850.036.973366119
TAAGTAG59800.035.88885516
TCCCAGG147300.035.83097530
CCAGGTT147650.035.7522332
CCCAGGT148650.035.46980731
TAAGGGG146900.035.3888771
TGTTACG57650.034.9088023
TTAGCGT61650.034.82914796
Produced by FastQC (version 0.11.5)