FastQCFastQC Report
Wed 5 Oct 2016
2_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685
Sequences flagged as poor quality0
Sequence length4-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGTCTGATGGAACGACGAA80.14072119613016712No Hit
TGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCCTTTTCA70.12313104661389623No Hit
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC60.10554089709762532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGAA100.008390243135.60214117
TTGTAAT100.008665814134.15958108
CAACAAG100.008665814134.15958116
TCGTCCA100.008947331132.74738105
Produced by FastQC (version 0.11.5)