FastQCFastQC Report
Wed 5 Oct 2016
2_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15614151
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT477770.30598525657911213No Hit
GTCAAACAGACGCGCAAGGGAGAAGATATCGTTGCCAGTACTTCTCTCGT179790.11514554970039677No Hit
CTGCCAGTAGTCATATGCTTGTTTCAAGGACTTAGCCATGCATGCCTCAG176000.1127182643487949No Hit
ACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCTCGAT160610.10286182066511333No Hit
GTTTGGTTGATTCCGTCAACGGACGAGATCCAAGCTGCCCAGTAGGATTC157520.10088284659217142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT249550.066.0746151
TTACCAG125000.046.573293
ACAGTTT362600.046.0358282
CCAGATC156500.037.2357256
CAGTTTC445150.037.1737943
TACCAGA156400.037.1108554
ACCAGAT158050.036.9460145
CTTTACC163200.036.452281
TCCGGAC164550.035.24530411
GTTTCTG925000.035.2313271
CAGATCC164800.035.220417
TTCTGTA985200.032.915493
TCTGTAC991750.032.668214
GATCCGG181600.032.1252379
ATCCGGA185300.031.67335910
TGTACTA1054000.030.7555186
CTGTACT1063400.030.452565
AGATCCG194000.030.0395418
TACTATA1079800.030.0059818
TTTCTGT1090850.029.913052
Produced by FastQC (version 0.11.5)