FastQCFastQC Report
Wed 5 Oct 2016
2_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8352
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCCGCGAAATCTCGTA150.17959770114942528No Hit
GCCCAACTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGA140.16762452107279693No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG130.15565134099616856No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT130.15565134099616856No Hit
CTGGACATAGTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACC110.13170498084291188No Hit
GTTTCTGTACTATATTGTATGACGCTGTCTTGGCGAGGAGCATGATGCGT90.10775862068965517No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG90.10775862068965517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGG100.0091298865132.31691117
CGTCCAC100.0091298865132.31691115
CCATCGC100.009926009128.69179105
TGTTCTT300.00510834456.2544915
Produced by FastQC (version 0.11.5)