FastQCFastQC Report
Wed 5 Oct 2016
3_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465
Sequences flagged as poor quality0
Sequence length4-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT80.10716677829872738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA100.008431459135.7295120
TGTAATA100.008431459135.7295120
AACACTG100.009292259131.42064104
Produced by FastQC (version 0.11.5)