FastQCFastQC Report
Wed 5 Oct 2016
3_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15650971
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACCAGATCCGGACAGGGTGAGGATTGACAGATTGAGTGTTCTTTCT186040.11886802422673969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT284850.064.155741
ACAGTTT406700.045.2916222
CAGTTTC496800.036.972563
GTTTCTG1030300.035.3228531
TTCTGTA1108750.032.7879453
TCTGTAC1119400.032.507674
TGTACTA1175000.030.946966
CTGTACT1187600.030.6411535
TACTATA1197000.030.393968
TTTCTGT1208750.030.2095742
GTACTAT1214150.029.9542397
ACTATAT1233100.029.5051889
CTATATT1281650.028.44709210
AGTTTCT655300.028.1187974
TTACCAG85150.027.8913
TATATTG1357900.026.84672511
ACATAAG65800.026.0597086
TCCGGAC91900.025.40738111
CATAAGG66350.025.0582227
ATATTGG484300.023.68911612
Produced by FastQC (version 0.11.5)