FastQCFastQC Report
Wed 5 Oct 2016
3_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename3_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10342
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCAATGACCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTG160.15470895378070004No Hit
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT140.13537033455811254No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG110.1063624057242313No Hit
CAAACCTCCACTGCCTCTGAAGCCCAACTGGACATAGTGGAAGGTTGTTA110.1063624057242313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACAGT100.00906097132.82082112
GTGCAGA100.0098711625129.08989101
TGTAGGG200.001045395684.0658517
AGGGAGG350.00978900147.80443610
Produced by FastQC (version 0.11.5)