FastQCFastQC Report
Wed 5 Oct 2016
4_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085977
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT17350.037.58729113
CTAGGTT18500.035.119194114
TAGGTTA22550.029.319134115
GCGCTAG24400.027.204527111
AGGTTAG24900.027.030483116
CGCTAGG26600.024.867672112
TAAGGGG51500.024.0432871
GGTTAGG28300.023.445593117
AGCTAAG27050.021.97513
AGTAAGG63750.019.75573369
CTAAGTA30600.019.42619315
TAGGGTA30950.019.39632420
GTAAGGG65650.019.11046270
CCCCGAT40850.018.9345591
CTAACGG23700.018.03808232
CCCTTAG36500.017.90944793
CCTATGG39700.017.74465667
CTAATCA55400.017.7065833
GGCGCTA36850.017.524775110
GCCGAAC72950.017.42274562
Produced by FastQC (version 0.11.5)