FastQCFastQC Report
Wed 5 Oct 2016
4_R1U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R1U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6427
Sequences flagged as poor quality0
Sequence length1-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTCCTCCTCCAAGAATTTGTGAAGAGTTTTGTGT130.20227166640734404No Hit
CTGCGCAGGATGTCCACTAATTCCGCCTGTGTCTGCAGGGCACCCAACTG100.1555935895441108No Hit
GCGTCTTTCCAGTCTCAGCAACTTCCCGTGTTCGTCTGATGGAACGACGA70.10891551268087755No Hit
CTCGTACACCTCATACGCATTCAGCTGACAAATAAAGGCAAATGTGAAAACAGAAAGGCC70.10891551268087755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG100.008144769137.06120
GCGGTGC100.008646574134.37254103
AGGCGGT100.008646574134.37254101
CGGTGCG100.008646574134.37254104
GTAGGCG100.008646574134.3725499
GTGCGGA100.008646574134.37254106
TCTTTAC100.008646574134.37254100
TAGGCGG100.008646574134.37254100
AACGTAG100.008904997133.0679596
ACGTAGG100.008904997133.0679597
CGTAGGC100.008904997133.0679598
CACGAAG100.008904997133.0679598
ACATCTG100.008904997133.0679597
CACAACG100.009168499131.7884593
ACAACGT100.009168499131.7884594
TGTCTCT100.009989965128.0934486
CACACAC350.00844579449.36134346
Produced by FastQC (version 0.11.5)