FastQCFastQC Report
Wed 5 Oct 2016
4_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15085977
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT267950.057.433841
ACAGTTT397150.039.069872
CAGTTTC461700.033.5704733
GTTTCTG936800.033.3420371
TTCTGTA1002100.030.9512923
TCTGTAC1007700.030.77974
TGTACTA1054900.029.3819776
CTGTACT1069050.029.024425
TACTATA1077850.028.747568
GTACTAT1094250.028.3523247
TTTCTGT1109250.028.1380622
ACTATAT1117850.027.7457249
CTATATT1165550.026.6509210
ACATAAG67250.026.5255956
ATACAGT114850.026.1379366
GATACAG119200.025.9580235
AGTTTCT600500.025.8305384
TGATACA121150.025.7782024
GATAAAC71000.025.528941
CATAAGG68050.025.4507567
Produced by FastQC (version 0.11.5)