FastQCFastQC Report
Wed 5 Oct 2016
4_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11243
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT180.16009961753980254No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG150.13341634794983545No Hit
GCAGGACATACCAGTGGTGTGTGGATCAGAATCTTCCGCGAAATCTCGTA140.12452192475317975No Hit
GTGGAAGGTTGTTATGTTCTTCCCGAGGGAAAAGACAGACCGTGTGTGGCATCGTTAAATTCTACTCCAGAG130.11562750155652406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGC100.008411033136.20879110
GGACGAG100.008691899134.72826107
GTTGACC100.008834637134.0105
CGGGAGA100.009124768132.56683102
TGAGGAC100.009421158131.1640296
GGGCCGT100.009723873129.7905788
TCGTGTA153.4502932E-4111.16592414
GATCGGA252.368361E-588.535711
GCGTCGT305.7206264E-574.1106211
TCGGAAG305.8500555E-573.779763
CGTCGTG351.227435E-463.5233812
ATCGGAA402.4292225E-455.334822
Produced by FastQC (version 0.11.5)