FastQCFastQC Report
Wed 5 Oct 2016
5_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16053098
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT14000.030.398054114
GCTAGGT14250.029.199116113
TAGGTTA19700.021.391619115
TAAGGGG44200.021.34121971
TGGACTA29250.018.318594118
GCGCTAG21800.018.230257111
CCTATGG40000.017.82739667
GGACTAC28850.017.468767119
AGGTTAG24650.017.207537116
CGCTAGG24050.016.62885112
AGCTAAG23550.016.48668513
TCCTATG42700.016.41857166
AGTAAGG58600.016.27964269
GGTTAGG27100.015.758868117
AGACCTA15050.015.75046997
TATCCTA44900.015.72791264
CCCCGAT45700.015.7263041
GTAAGGG59950.015.62931370
CTAAGTA26750.015.16533915
CGAACTA36400.015.1223374
Produced by FastQC (version 0.11.5)