FastQCFastQC Report
Wed 5 Oct 2016
5_R2P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16053098
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTT306150.059.94281
ACAGTTT433750.042.6834682
CAGTTTC529150.034.8353463
GTTTCTG1053350.033.0880851
TTCTGTA1134350.030.5881293
TCTGTAC1146350.030.2935984
TGTACTA1198350.028.9748636
ATACAGT122350.028.910076
TGATACA124000.028.7118054
CTGTACT1214500.028.5619855
TACTATA1221900.028.4094988
TTGATAC126000.028.3477483
TTTCTGT1244750.028.0656572
GTACTAT1237700.028.0642627
ACTATAT1261150.027.5406679
GATACAG129750.027.530425
AGTTTCT688600.026.8198434
CTATATT1311150.026.54072810
ACATAAG64450.025.5516346
CATAAGG63700.025.3074047
Produced by FastQC (version 0.11.5)