FastQCFastQC Report
Wed 5 Oct 2016
5_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12303
Sequences flagged as poor quality0
Sequence length1-126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGGCTCCATGGGACGCTGCCCGTGCAGCGTTTGAATCAAACCT160.13004958140290984No Hit
CTCGTATTGTGCTTCCTGTCACACTCAATGACCTCGATCGGCTCCATGGG150.121921482565228No Hit
CCGCAAATGACGGGGCGGCCAGATGCACGCGAGGCGGTGGCACAATACTG140.11379338372754612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAT100.009538572130.69952113
TTTGCTA100.009538572130.69952113
TTGCTAC100.009538572130.69952114
AACGACT100.009673671130.08878110
TGGCACT100.009947669128.88426100
ATCTACA100.009947669128.88426103
ACATCCA100.009947669128.88426103
TAACACA100.009947669128.88426101
Produced by FastQC (version 0.11.5)