FastQCFastQC Report
Wed 5 Oct 2016
6_R1P.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename6_R1P.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15371310
Sequences flagged as poor quality0
Sequence length1-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCC507580.3302125843535782No Hit
CCCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCG319290.20771814503773586No Hit
CCCCGAACTACCCTCCTTCATTCCTGGATGCCGTGAGTTGAGGGAAGGCA249780.16249753599400443No Hit
ACCGCACGCTCCACGTCTTGTGGTGCCATTCCGTCAATTTCTTTAAGTTT244430.15901702587482783No Hit
GTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACTG240590.1565188653406899No Hit
CTTCGCCAAATATGAGACCACACGAAGAAGAAGGAGCGCTTTGTCCCTCC223330.1452901541898511No Hit
CTCGCAATTTCCAGCTAAGTAGGGTAAAACCAACCTGTCTCACGACGGTC217570.14154291338864416No Hit
GTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTC217240.1413282277177417No Hit
CTCTAATCATCTTCGATCCCCACACTTTGGTTCTTGATTGAGGAAGGTAT217200.14130220521217776No Hit
CCGCAGAGAAGGATACAAGCGAATACCGCCCCCCCTTGGGTAAAACCCGC210470.13692391865104536No Hit
ACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCGGTCAAG207800.13518691640465255No Hit
CCCTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTG194990.12685320899780173No Hit
CTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAACTTTCG194100.12627420824900415No Hit
CTGAGACTGTAACCTCAAAGCTTTCGCGTGAAGAAGTCAGATAACGTGCT193760.1260530169517107No Hit
CGTAAATTTAATATATTCATTGGCGAGACGAGGAACGGGATGAAAAATAA192370.12514873488336387No Hit
CCACATGCAATTTCTTTTTGGAATATAATCCCAGGTTCTCTGTTGCCCCT166880.10856589321274505No Hit
CGTCAATTTCTTTAAGTTTCACTCTTGCGAACGTACTCCCCCCTGAGACT158390.10304261640679943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT50850.057.266533113
CTAGGTT51800.056.831764114
TAGGTTA54400.054.21759115
AGGTTAG58600.050.548126116
GCGCTAG58000.049.929863111
CGCTAGG58250.049.790615112
GGTTAGG63100.047.47524117
AGCTAAG62000.045.12011313
TTAGGAA68850.043.46758119
GGCGCTA67200.043.0245110
CTAAGTA65300.042.93145815
CCCTTAG68300.042.10222293
TAGGAAG71650.042.043774120
TAGGGTA67650.041.70131320
TAAGTAG72800.039.1467816
TTAGCGT74400.038.7622396
TGTTACG58900.038.6246383
AGTAGGG74150.038.5918718
CCCACAT179950.038.422691
CAGCTAA75350.037.4336512
Produced by FastQC (version 0.11.5)