FastQCFastQC Report
Wed 5 Oct 2016
7_R2U.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename7_R2U.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660
Sequences flagged as poor quality0
Sequence length1-126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTG100.009455905130.9837119
CGCGTGT100.009455905130.9837120
GGTGCTT100.009455905130.9837119
ACGCCCA100.0096111735130.27568114
GGGAGTA100.0096111735130.27568118
GCGTATC100.0096111735130.27568116
ATTGCGA100.009926772128.88235102
GCGTCTC100.009926772128.88235104
AGCAGTC152.1628581E-4124.8756588
TACAGTT153.2117037E-4113.150231
AGTTTCT153.2117037E-4113.150234
TCGTGTA402.0918378E-1099.00645414
GGAAAGA304.3824548E-794.7366322
AGTCCGG206.5153366E-494.63743691
CGTCGTG304.508529E-794.29185512
CAGTCCG206.653078E-494.1445390
GCGTCGT252.1167345E-590.52019511
AAACCAC209.5243426E-486.07500524
GTCGTGT402.447996E-884.8626713
GGGAAAC200.001008045584.8626721
Produced by FastQC (version 0.11.5)